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KMID : 1143420200130473356
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Public Health Weekly Report
2020 Volume.13 No. 47 p.3356 ~ p.3363
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Multilocus sequence typing analysis of Mycoplasma pneumoniae strains isolates in Korea, 2006-2019
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Kim So-Hyeon
Kim Dong-Hyeok
Chun Jeong-Hoon
Hwang Kyu-Jam
Jung Sang-Oun
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Abstract
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It is generally accepted that Mycoplasma pneumoniae (M. pneumoniae) induces both upper and lower respiratory infections and is responsible for about 20-40% of community-acquired pneumonia. M. pneumoniae infection occurs endemically, with epidemic peaks every three to four years, mostly in children over five years of age. Recent studies indicated that a critical issue is the emergence of antibiotic resistant strains and an increase in the ratio of distribution strains. Therefore, this study hypothesized that a genotype analysis of strains would be useful to determine and monitor the cause and the epidemic patterns of an outbreak.
Multilocus sequence typing (MLST) is routinely performed for strain differentiation in many genera of bacteria, and is useful for strain differentiation of M. pneumoniae. This article determined that MLST method was used to confirm the molecular epidemiologic relationship between epidemic patterns and antibiotic resistance in 127 M. pneumoniae strains isolated in Korea from 2006 to 2019. This study found that the age distribution of the strains was highest among children aged 0 to 9 years (59.8%, 76 cases) and 10 to 19 years (17.3%, 22 cases).
The major MLST genotypes detected in the housekeeping genes were ST3 (53.5%), ST14 (31.5%) and 4 minor genotypes were also detected (ST2, ST7, ST20, ST33). Moreover, a new sequence type(ST) was observed in 2018 as 0.8% of frequency and ST33 was also observed in 2019 as 2.4% of frequency. According to this article’s comparison of the STs of M. pneumoniae strains identified with macrolide-resistance gene mutation (23S domain V, A2063G), the frequency of ST3 (18, 66.7%), ST14 (3, 11.1%), ST20 (3, 11.1%), ST7 (1, 3.7%), ST33 (1, 3.7%), and NT(1, 3.7%) was confirmed. This study confirmed that the distribution of STs and the genotype of M. pneumoniae in Korea are changing.
Therefore, continuous respiratory infection monitoring is necessary to predict and control the prevalence and strains characteristics of M. pneumoniae in Korea. This study further recommended the use of antibiotic sensitivity tests and additional characterization analyses of strains and new ST type identification methods.
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KEYWORD
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Mycoplasma pneumoniae, MLST, Genotype, Macrolide, Antibiotic resistance gene mutation, NGS
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